Journal: Seminars in Immunopathology

Article Title: The role of the aryl hydrocarbon receptor in normal and malignant B cell development

doi: 10.1007/s00281-013-0390-8

Figure Lengend Snippet: Relative AhR mRNA expression in purified subpopulations of murine B cells. Microarray data were generated by Green et al. from murine B cells sorted by flow cytometry based on B developmental stage-specific surface antigens. Expression levels of the AhR transcript within the listed differentiation stages was extracted from and the corresponding distributions summarized and displayed as 'box-and-whiskers' plots (with the bottom and the top of the box corresponding to the first and third quartiles, the thick band inside the box indicating the median, and the end of the 'whiskers' extending to 1.5 times the interquartile range in both directions)

Article Snippet: Microarray analysis of 1,036 human cancer cell lines generated at the Broad Institute, i.e., the Cancer Cell Line Encyclopedia ( http://www.broadinstitute.org/ccle/home ) (Fig. ), revealed a hierarchy of AhR expression in which low levels of AhR were expressed in diffuse large B cell lymphomas, unspecified B cell lymphomas, and myelomas and notably higher levels in Hodgkin’s lymphomas and chronic lymphocytic leukemias (Fig. ).

Techniques: Expressing, Purification, Microarray, Generated, Flow Cytometry

Journal: Seminars in Immunopathology

Article Title: The role of the aryl hydrocarbon receptor in normal and malignant B cell development

doi: 10.1007/s00281-013-0390-8

Figure Lengend Snippet: AhR mRNA expression varies among five human B lineage cancers. Relative levels of AhR expression in five human B lineage cancers were assessed by analysis of microarray data obtained from 1,036 human cancer cell lines ( http://www.broadinstitute.org/ccle/home ). Each box plot reports the distribution of the AhR transcript within the samples belonging to the corresponding B lineage cancer type

Article Snippet: Microarray analysis of 1,036 human cancer cell lines generated at the Broad Institute, i.e., the Cancer Cell Line Encyclopedia ( http://www.broadinstitute.org/ccle/home ) (Fig. ), revealed a hierarchy of AhR expression in which low levels of AhR were expressed in diffuse large B cell lymphomas, unspecified B cell lymphomas, and myelomas and notably higher levels in Hodgkin’s lymphomas and chronic lymphocytic leukemias (Fig. ).

Techniques: Expressing, Microarray

Journal: Seminars in Immunopathology

Article Title: The role of the aryl hydrocarbon receptor in normal and malignant B cell development

doi: 10.1007/s00281-013-0390-8

Figure Lengend Snippet: Relative expression of AhR mRNA and AhR target genes in human tumor cell lines. Analysis of microarray data obtained from 1,036 human cancer cell lines ( http://www.broadinstitute.org/ccle/home ) is presented. a Data corresponding to the five lymphoid malignancies listed and five transcripts, including AhR and four of its putative targets, are displayed as a color-coded gene-by-sample heat map, with rows (genes) and columns (samples) sorted by hierarchical clustering . b Microarray data for the same five lymphoid cancers were analyzed, and genes ranked by Pearson correlation between the level of AhR expression and that of four known AhR target genes, CYP1B1 , NQO1 , TIPARP , and AhRR . Permutation-based p values and the corresponding FDR-corrected q values are shown. c A Kolmogorov–Smirnov test was performed to assess the strength of the association between AhR and its four targets. The x -axis lists the genes in the human transcriptome sorted by their distance from AhR (from the closest, left , to the furthest, right ). The position of the four AhR targets ( red ticks ) is significantly skewed toward the left-hand side of the list (permutation-based p value = 0.021)

Article Snippet: Microarray analysis of 1,036 human cancer cell lines generated at the Broad Institute, i.e., the Cancer Cell Line Encyclopedia ( http://www.broadinstitute.org/ccle/home ) (Fig. ), revealed a hierarchy of AhR expression in which low levels of AhR were expressed in diffuse large B cell lymphomas, unspecified B cell lymphomas, and myelomas and notably higher levels in Hodgkin’s lymphomas and chronic lymphocytic leukemias (Fig. ).

Techniques: Expressing, Microarray